XF-1600™ from Sysmex India Pvt. Ltd.

Flow cytometry (FC) is a technology to analyze light scattering and fluorescent optical properties of single particle in liquid suspension under high pace fluidics stream through a beam of light, usually a laser, to facilitate discrimination, quantification, and sorting of discrete cell population, based on simultaneous analysis of multiple physical parameters. FC offers a wide range of applications in the clinical, pharmaceutical, microbial, plant, and animal sciences by revealing properties of individual cell. Recent advancement in laboratory-developed tests (LDTs) for simultaneous multiple antigen screening extends a complex methodology and data analysis.

Sysmex is an aggressive player in multiple castigations including hematology, urinalysis, and hemostasis and a benefactor in automation workflow. Sysmex has now introduced a next level of automation of flow cytometry. Its flow cytometry system comprises PS-10 sample-preparation system, Rotolavit-II-S automatic cell-washing centrifuge, XF-1600™ flow cytometer, Abs portfolio, ancillary reagents, and VenturiOne data analysis software.

The PS-10 sample preparation system improves the primary bottleneck in today’s busy clinical flow cytometry laboratory, while providing outstanding flexibility for the creation of complex LDT applications. PS-10 is a smart autosampler with LIS compatibility for high-throughput sample processing of up to 50 specimens. Its multiple and integrated barcode readers automatically detect sample ID of tube and rotors to reduce operator errors. Dual pipetting probes design and dual 24-tube rotor maximize workflow efficiency. Sample preparation reagent blocks are designed to conveniently accommodate up to 90 monoclonal antibodies (mAb) combination of vials for cocktail creation. PS-10 rotors are directly compatible with the Rotolavit II-S (automatic cell-washing system), which for wash procedures, virtually eliminate daughter tube handling and human errors when transferring tubes to and from a centrifuge for washing. The Rotolavit II-S is fully programmable for automatic wash cycles, decanting, and resuspension of cell pellets.

Sysmex is also introducing a wide range of Mab for clinical applications with feature of a convenient, readable QR code for automatic entry of reagent type, product code, lot number, and expiry date.

The XF-1600™ is 3L, 10C, 37-parameter flow cytometer for in vitro diagnostic use and innovation of integration between multi-laser optical layout with Sysmex’s fluidics design for reliable performance. Its stable fluidics – even at high sample acquisition rates – ensurerapid data collection without loss of resolution.

The XF-1600™ uses a streamlined quality-control setup with an easy-to-read radar, and Levy-Jennings charts ensure quality data. Samples can be loaded on a variety of tube sizes, with selectable aspiration volumes to ensure minimal sample waste and on a universal Sysmex rotor for automated walk-away acquisition.

For additional convenience, universal rotors can easily be moved between PS-10 sample-preparation system, automated cell washer/centrifuge, and XF-1600 flow cytometer. By monitoring barcodes, rotors are positively identified to ensure traceability throughout the laboratory, and better workflow efficiency with no need for creation of a work list while connecting with XF-1600™. Automated sample preparation and controlled cell washing guarantee excellent cells viability, cells recovery, and reproducible results.

With the growing interest in rare-event analysis, the XF-1600™ incorporates features of rare-event analysis mode (REM). The system allows users a choice of five adjustable sample rates – from 5 µL/min to 120 µL/min, which utilize up to 2 mL of specimen for analysis, while ensuring low carryover and a low limit of blank (LoB). At high sheath velocity, fast data acquisi­tion rates – up to 50,000 events per second – reduce the overall sample-acquisition time, while maintaining the essential sensitivity required for rare event analysis. Merging FCS files, simultaneous threshold, and maintaining higher singlet-doublet features of REM offers highly sensitive analysis of rare events.

In conclusion, highly trained operators no longer need spending hours manually pipetting, leaving their valuable time for more complex analytical activities.